HP Data Explorer 4 Creating extracted ion chromatograms

Mariner Data Examples

The combined spectrum is displayed (Figure 7-3), with two intense peaks at 410 Da and 723 Da. Generate extracted ion chromatograms as described below to determine if these peaks are the coeluting components.

NOTE: If these peaks do not yield chromatograms with profiles that correspond to the unresolved peaks, try another spectral peak.

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Figure 7-3 Combined Spectrum for Unresolved Peaks in Cytochrome C

Creating extracted ion chromatograms

To create extracted ion chromatograms:

1. With the Chromatogram window activated, click in the toolbar two times to add two traces. Click an added trace to activate it.

2.In the Spectrum window, right-click-drag over the first peak.

The extracted ion chromatogram is displayed.

3.In the Chromatogram window, click the second added trace to activate it.

4.In the Spectrum window, right-click-drag over the second peak.

The extracted ion chromatogram is displayed.

Figure 7-4illustrates the peaks deconvoluted from the original unresolved peaks.

Data Explorer™ Software User’s Guide 7-5