Chapter 6 Using Tools and Applications

6

 

6.3 Using the

 

Mass Resolution Calculator

 

 

 

 

 

 

 

 

Calculating mass To calculate mass resolution:

 

 

 

resolution 1. Display the spectrum of interest. Make sure the

 

 

 

Spectrum window is active.

 

 

2. If you are examining Voyager data, baseline correct the

 

 

 

spectrum. The Peak Height used by the Resolution

 

 

 

calculator is calculated from 0. For information, see

 

 

 

Section 5.8.2, Using Baseline Correction.

 

 

 

 

 

 

 

NOTE: Baselines in Mariner data are typically at 0.

 

 

 

Baseline correction is not necessary.

 

 

 

 

3. From the Tools menu, select Resolution Calculator.

NOTE: If the Spectrum window is not active, Resolution

Calculator does not appear on the Tools menu.

4. In the Resolution Calculator dialog box (Figure 6-12),set the percentage of peak height at which to calculate resolution. The default is 50%, which calculates the resolution at the full width/half maximum (FWHM) of a peak.

NOTE: Measuring resolution at the full width/half maximum is the industry standard. Use 50% Peak Height for most applications. If a peak is not resolved at the Peak Height % you set, an error message is displayed in the Result tab.

6-20 Applied Biosystems