Appendix C Data Explorer Toolbox (Visual Basic Macros)
C
2.If desired, click Label Immonium Ions.
NOTE: Label immonium ions before selecting a reference peak and starting the search. If you click Label Immonium Ions after selecting a reference peak, the amino acid labels applied to the spectrum are erased, and mass labels are reapplied.
3.Select a peak in the Spec Peak List, then click Set Selected Peak as Reference.
The software evaluates mass differences between the reference peak (labeled with *) and all other peaks, and labels any differences that correspond to amino acid residues.
4.Select a result peak from the results list, if desired, then continue the sequence search by clicking the Search Down or Up buttons.
The newly selected peak is now labeled as the reference peak, and mass differences minus (if you click Down) or plus (if you click Up) are labeled if they correspond to an amino acid residue. You can generate the complete sequence ladder by appending new results to existing results.
NOTE: When you search using reference peaks selected from the Sequence Results list, new results retain the label of the original reference peak, and new labels are appended. For example, if you select 433.08(+Thr)(*) as the reference, results are listed as