Chapter 4 Examining Chromatogram Data

4.1 Overview
Types of Mariner data

This section includes:

Types of Mariner data

Types of Voyager data

Creating macros to combine processing functions

Returning to the original trace

You can display different types of chromatogram traces from Mariner data files by selecting Traces from the Display menu with the Chromatogram window displayed, then selecting:

 

 

Select

To display

 

 

 

 

 

 

TIC

Total Ion Chromatogram, which includes the entire mass range

 

 

 

saved in the data file. Each data point represents the sum of all ion

 

 

 

intensities in the corresponding spectrum.

 

 

 

 

 

 

BPI

Base Peak Intensity trace, which includes only the base peak in

 

 

 

each spectrum. Each data point represents the single most intense

 

 

 

ion in the corresponding spectrum.

 

 

 

 

 

 

Analog

Trace of the input from an outside source representing any signal

 

 

 

 

that changes over time, for example, the UV signal from an

 

 

 

LC system, or the Analyzer Temperature, Air Temperature, Spray

4

 

 

Tip Potential, or Nozzle Temperature from the Mariner mass

 

 

spectrometer.

 

 

 

 

 

 

DAD TAC

Diode Array Detector (DAD) Total Absorbance Chromatogram

 

 

 

(TAC), which displays data acquired from LC samples.

 

 

 

Hint: To display spectra for DAD data, double-click the TAC or

 

 

 

 

 

 

Ch Absorbance trace.

 

 

 

 

 

 

DAD

Diode Array Detector Channel chromatograms, which display

 

 

Channel N

specific wavelength ranges, where N is the channel number set

 

 

 

during acquisition. The data file can include up to five channels.

 

 

 

Hint: To display spectra for DAD data, double-click the TAC or

 

 

 

Ch Absorbance trace

 

 

 

 

4-2 Applied Biosystems