Chapter 5 Examining Spectrum Data

5.4 Automatic Calibration

This section includes:

Overview of automatic calibration

Importing and specifying automatic calibration settings

Automatically calibrating (Mariner data only)

NOTE: Automatic calibration is not supported for

Mariner DAD data.

5.4.1 Overview of Automatic Calibration

During automatic calibration

5

This section includes:

During automatic calibration

Automatic calibration for Mariner data

Automatic calibration for Voyager data

During automatic calibration:

You specify auto calibration settings (reference masses, matching criteria, and fit rejection parameters). Auto calibration settings are saved as part of processing settings in a .DAT file.

If Auto Calibrate is enabled in the Data Explorer software (Mariner data only), or if the Automatic Calibration function is accessed by the Voyager Sequence Control Panel (Voyager data only), the software does the following for each spectrum it displays or processes:

Compares peaks in the trace to peaks listed in the Masses to Match list in auto calibration settings.

5-26 Applied Biosystems