Chapter 5 Examining Spectrum Data

5.5 Centroiding

NOTE: Centroiding is not supported for Mariner DAD data.

To display peaks as centroid traces:

1.Click the Spectrum window to activate it.

2.Select Duplicate Active Trace from the Display menu to keep the original data displayed after processing.

3.From the Process menu, select Centroiding.

The centroid spectrum is displayed with a CT trace label (see Figure 5-10). The height of each vertical bar corresponds to the original peak area.

Figure 5-10 Centroid Spectrum

4.To return to the original trace, see “Returning to the original spectrum” on page 5-3.

NOTE: If the original spectrum is displayed in vertical bars instead of lines, select Graphic Options from the

Display menu, click the trace tab of interest, then select Lines for the Line Type in the Plot Setup section of the

5dialog box. For more information, see Section 1.5, Setting Graphic Options.

5-36 Applied Biosystems