Chapter 4 Examining Chromatogram Data

Example Figure 4-4shows a TIC that contains three flavonoid compound peaks. To determine if the diglycosyl group has fragmented from the parent ion in any of these compounds, you can generate a CNL extracted chromatogram.

Figure 4-4 TIC for Flavonoid Mixture Containing Three Peaks

Figure 4-5shows a CNL extracted chromatogram generated for mass difference of 308.146 m/z which corresponds to the diglycosyl group.

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Figure 4-5 CNL Containing Two Peaks

The CNL extracted chromatogram contains only two peaks, indicating that the diglycosyl group has fragmented from the parent ion in the first two compounds only.

4-12 Applied Biosystems