Chapter 5 Examining Spectrum Data

5.4.3Automatically Calibrating (Mariner Data Only)

Automatically calibrating

This section includes:

•Automatically calibrating

•Applying new constants to the data file

•Calibration results

•Applying auto calibration settings to other files

To automatically calibrate:

1.Open the data file to calibrate.

2.Click the Spectrum window.

3.From the Process menu, select Mass Calibration, select Auto Calibrate State, then select On.

 

 

 

NOTE: The Auto Calibrate State command setting is

 

 

 

stored in a .DAT file. That is, if you turn on this

 

 

 

command, then close the .DAT file, the command is on

 

 

 

the next time you open the .DAT file.

 

 

 

 

 

 

 

 

 

 

 

NOTE: The Auto Calibrate State command is dimmed

 

 

 

unless you specified automatic calibration settings in

 

 

 

the data file. See Section 5.4.2, Importing and

 

 

 

Specifying Automatic Calibration Settings.

 

 

 

 

4. Display the spectrum of interest.

 

 

 

The spectrum is calibrated and displayed with an AC

 

 

 

 

 

 

trace label if calibration is successful. Calibration

5

 

 

results are displayed in the Output window.

 

 

If calibration fails, the spectrum is displayed with an

 

 

 

AC-failed trace label.

 

 

 

 

5-34 Applied Biosystems