Chapter 3 Peak Detection and Labeling

Example Figure 3-19and Figure 3-20illustrate the effects of deisotoping.

Before deisotoping (Figure 3-19),the spectrum includes an isotope pattern with four detected peaks.

 

 

Figure 3-19 Spectrum Before Deisotoping

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After deisotoping (Figure 3-20),the trace includes two

 

labeled centroid bars that represent monoisotopic masses,

 

 

 

 

indicating that the original trace represents two isotopic

 

 

envelopes. The intensity of a centroid bar is proportional to

 

 

the area of all peaks in the isotope cluster.

 

 

 

 

 

 

 

 

 

 

 

 

Figure 3-20 Spectrum After Deisotoping

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