Chapter 5 Examining Spectrum Data

Manually matching peaks

6.Select the Peak Weighting Factor. If the calibration includes more than two points, you can apply the following weighting factors to fit points to the curve:

•None— All peaks weighted equally

•Inverse Width— Narrower peaks are weighted more than broader peaks

•Height— More intense peaks are weighted more than less intense peaks

7.To manually select the reference mass for a peak, right-click-drag over the peak of interest.

The Select or Create Reference Peak Information dialog box (Figure 5-3)is displayed and lists all masses in the selected reference file. The selected entry is the nearest match in the calibration reference file to the selected peak.

5

Figure 5-3 Select or Create Reference Peak

Information Dialog Box

5-10 Applied Biosystems