Chapter 5 Examining Spectrum Data

Eliminating data points

9.Repeat step 7 and step 8 until all desired peaks are in the matched list.

10.To eliminate unacceptable data points from the calibration, do either of the following:

•Select the data point (mass) in the Peaks matched list, then click Delete Selected Match.

•Click Eliminate Fit Outlier. The software deletes from the calibration the data point with the largest calibrated Fit Error (difference between the calibrated mass and the reference mass) as reported in the Output window (Figure 5-4).

NOTE: The point deleted may not be the point with the largest Initial Error (difference between the pre-calibration observed mass and the reference mass) listed in the Peaks Matched List in the Manual Calibration dialog box.

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5-12 Applied Biosystems