HP Data Explorer 4 5.3.3Creating or Modifying a Calibration Reference File (.REF)

Manual Calibration

5.To save the calibration to the data file, select Mass Calibration from the Process menu, then:

If you are	Select	The following occurs
calibrating
Mariner	Apply Calibration	All spectra in the data file are calibrated and
data		displayed with an MC trace label. The
(including		calibration constants are saved with the data
MS Method		file. Each spectrum in the data file is
data)		calibrated when displayed.
Voyager	Apply Calibration	The current spectrum is calibrated and
data		displayed with an MC trace label. The
		calibration constants are saved with the
		spectrum.
	Apply to All	All spectra in the data file are calibrated
	NOTE: This button is	using the currently displayed calibration, and
	displayed only if you	are displayed with an MC trace label. The
		calibration constants are saved with the data
	are calibrating a
		file. Each spectrum in the data file is
	Voyager
		calibrated when displayed.
	multispectrum data
	file.

5.3.3Creating or Modifying a Calibration Reference File (.REF)

This section includes:

•Definition

•.REF files provided

• Calibration reference file contents		5
•	Creating and saving a calibration reference file
• Modifying a calibration reference file
•	Specifying mass type for highly charged, narrow peaks

Data Explorer™ Software User’s Guide 5-17