Chapter 5 Examining Spectrum Data

3.Enter parameters as described below. These parameters interact with each other and require experimentation to determine the optimum settings for your data. Refer to “General guidelines for setting parameters” on page 5-54for more information.

Parameter

 

Description/Specifies

 

 

 

 

 

 

 

Peak Width

Value the software uses to estimate the baseline

 

 

(at half height)

amplitude at regularly spaced points in the spectrum. The

 

number of regularly spaced points used is derived using

 

the Peak Width parameter and the Flexibility parameter.

 

The illustration below shows how using a higher number

 

of points estimate the baseline (accomplished by using

 

higher Peak Width and Flexibility settings) can affect the

 

shape and slope of the calculated baseline.

 

 

 

 

 

 

 

 

Lower number of points

 

 

 

 

x

x

x

x

Higher number of points

x x

x x

(continued)

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5-50 Applied Biosystems