Using the Ladder Sequencing Toolbox

NOTE: If the modToolBoxPalette.Toolbox_Palette is not listed, you must import the macro into the Data Explorer project. For information, see Section 6.7.7, Importing or Exporting Macros in DATAEXPLORER.VB6.

Hint: You can assign the

modToolBoxPalette.Toolbox_Palette macro to a macro button in the Data Explorer software. For information, see the Data Explorer User’s Guide, Section 6.7, Using the Macro Recorder.

You can also access individual macros directly, or assign individual macros to a macro button, by selecting the individual macro in the Macros dialog box.

C.4 Using the

C

Ladder Sequencing Toolbox

 

 

 

 

 

 

Use the Ladder Sequencing toolbox when you perform

 

 

 

 

sequencing to label peaks with the appropriate amino acid,

 

 

DNA, or RNA residue. Labels are determined by calculating

 

 

the mass differences between the peaks and comparing the

 

 

values to an internal listing of mass differences and

 

 

corresponding residues.

 

Running the

To run the Ladder Sequencing macro:

 

macro

1. In the Toolbox Palette dialog box, click Ladder

 

 

 

Sequencing Toolbox. The range displayed in the

Spectrum window is reflected in the Mass Range fields.

NOTE: If you zoom on a different region in the Spectrum window, the new range is not updated in the Mass Range field, but is used for the analysis when you click Label Peaks. The range is updated when you click any button.

Data ExplorerSoftware User’s Guide

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