HP Data Explorer 4 manual 382

Chapter 9 Troubleshooting

Table 9-12 Charge State and Isotope Determination Troubleshooting—Mariner

Only (Continued)

	Symptom	Possible Cause	Action
	Spectrum peaks not	Mass of original molecule	No action. Normal
	labeled with charge state	above 4,000 Da, not	occurrence.
	when charge state labels	range in which the Mariner
	are selected	system can resolve the
		isotope peaks
		Charge State parameters	Adjust parameters. See
		not set to detect at least	“Peak Processing
		two isotope peaks	parameters (spectrum
			data only)” on page 3-26.
		Peaks are more than 1 Da	No action. Normal
		apart	occurrence.
		Peaks are not from the	No action. Normal
		same isotope species	occurrence.
		Filter width is set too high	Adjust Filter Width. See
		to detect other isotope	“Advanced Settings
		peaks	(spectrum data only)” on
			page 3-28.
9
		Maximum Charge State	Adjust the Maximum
		for charge state	Charge State. See “Peak
		determination is set lower	Processing parameters
		than the charge state of	(spectrum data only)” on
		the peak	page 3-26.

9-20 Applied Biosystems