Chapter 7 Data Explorer Examples

7.2.2Processing Before Calibrating to Optimize Mass Accuracy

This section includes:

 

 

 

• Calibrating without baseline correcting and deisotoping

 

 

 

•

Before calibrating

7

 

 

•

Calibrating

Calibrating

For optimum mass accuracy, baseline correct and deisotope a

 

without baseline

spectrum before calibrating.

 

correcting and

Figure 7-12shows a spectrum before calibration.

 

deisotoping

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Figure 7-12 Spectrum Before Calibration

Figure 7-13shows a spectrum that has been calibrated without initial baseline correction and deisotoping. Mass accuracy is not acceptable.

Figure 7-13 Calibration Without Baseline Correction

or Deisotoping

7-14 Applied Biosystems