Appendix C Data Explorer Toolbox (Visual Basic Macros)

3.Add peaks to the peak list to be included in the calculation by typing a mass in the Add Mass to Peak List field, then clicking Add Peak.

Remove unwanted peaks from the list that you do not want included in the calculation by clicking the peak in the list, then clicking Delete Selected Peaks.

4.If you will be identifying y and b pairs, select the precursor peak, then click Use Selected Peak.

Pairs To list ion pairs:

1.Click the Pairs tab.

C

Figure 3-11 Peptide FragmentationPairs

2.Click the button that corresponds to the ion masses you want identified:

•a and b pairs Lists peak pairs with a 28 Da mass difference.

•(a, b, y) –17 pairs Lists peak pairs with a 17 Da mass difference, which corresponds to the loss of NH3.

C-10 Applied Biosystems