Chapter 5 Examining Spectrum Data

2.From the Peaks menu, select Peak Label, then set the Mass Label Type to Centroid.

NOTE: For spectra containing broad peaks that have unresolved adducts or impurities such as proteins, you may obtain better results if you use apex instead of centroid settings.

3.From the Process menu, select Mass Calibration, then select Automatic Calibration.

The Automatic Calibration Settings dialog box is displayed (Figure 5-7).

Figure 5-7 Automatic Calibration Settings Dialog Box

 

4. Click Select New File, then select a calibration reference

 

file. For information on creating a reference file, see

5

Section 5.3.3, Creating or Modifying a

Calibration Reference File (.REF).

 

 

 

5-30 Applied Biosystems