Chapter 3 Peak Detection and Labeling

 

 

Table 3-1 Chromatogram Settings (Continued)

 

 

 

 

 

 

 

 

 

 

Parameter

Description

 

 

 

 

 

 

 

 

 

 

Detection Ranges

To delete a range, select the range, then click

.

 

 

 

(continued)

To combine all ranges in the list into one range, click

.

 

 

 

The peak detection settings displayed in the dialog box

 

 

 

 

correspond to the selected range. To view peak detection

 

 

 

 

settings for another range, select the range of interest.

 

 

 

 

 

 

 

 

%Base Peak

Specifies a percentage of the base peak intensity as the

 

 

 

Intensity

threshold value.

 

 

 

 

NOTE: This

To be detected, peaks must be above this threshold and

 

 

 

parameter was

above the %Max Peak Area value.

 

 

 

 

previously named

Hint: For most Voyager applications, leave this parameter

 

 

 

Peak Threshold%

set at 0 and adjust the %Max Peak Area.

 

 

 

 

and Base Peak

 

 

3

 

 

 

 

 

Relative%.

 

 

 

 

 

 

 

 

 

%Max Peak Area

Specifies a percentage of the peak with the largest area as

 

 

 

 

 

the threshold value.

 

 

 

 

 

To be detected, peaks must be above this threshold and

 

 

 

 

above the %Base Peak Intensity value.

 

 

 

 

 

 

 

 

 

 

%Max Peak Area is calculated above the local baseline and

 

 

 

can compensate for problems related to a rising global

 

 

 

 

baseline and signal spikes.

 

 

 

 

 

Hint: If you are analyzing digest data, set %Max Peak Area

 

 

 

to 0 before deisotoping to ensure that all peaks of interest

 

 

 

 

are detected. For more information, see Section 7.2.3,

 

 

 

 

Detecting Peaks from Complex Digests.

 

 

 

 

 

 

 

 

 

Filter Width

Specifies the number of data points used in smoothing for

 

 

 

 

peak detection before integration. The software uses a Filter

 

 

 

Width Increment of 1 when applying smoothing.

 

 

 

 

 

NOTE: If you set Filter Width too high, narrow peaks may not

 

 

 

be detected.

 

 

 

 

 

 

 

 

 

 

 

Continued

3-20 Applied Biosystems