Chapter 8 Viewing Voyager PSD Data

8.3.3Creating PSD Calibration (.CAL) Files and Applying to Other Data Files

Creating PSD.CAL files

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Applying new constants to additional files

To generate a PSD .CAL file:

1.Acquire a standard, for example, angiotensin, in the Instrument Control Panel in PSD mode. For more information, see the Voyager Biospectrometry Workstation User’s Guide.

2.Open the .DAT file in the Data Explorer software.

3.Calibrate as described in Section 8.3.2, Calibrating.

4.From the File menu, select Export, then select Calibration.

5.Name and save the file.

Hint: Include a _PSD suffix when you export a PSD

.CAL file to help you distinguish them from non-PSD

.CAL files. For example, type Cal_PSD as the file name. The complete file name will be Cal_PSD.CAL.

You can apply calibration constants from a .CAL file to any data file. To apply the new constants from a mass calibration file to a different file:

1.Display the spectrum to calibrate.

2.From the Process menu, select Mass Calibration.

3.Select Import Calibration.

4.Select the .CAL file to use. Click Open.

The software displays the calibrated spectrum with an MC trace label.

5.To save the calibration to the data file, select Mass Calibration from the Process menu, then select Apply Calibration.

8-20 Applied Biosystems