Chapter 2 Using Chromatogram and Spectrum Windows

2.4.8 Overlaying Traces

This section includes:

2 Overlaying traces from different data files

•Overlaying traces from different data files

•Overlaying traces in a single data file

•Changing the active trace

•Sequentially activating overlaid spectra

•Setting overlay attributes

To overlay traces from different data files:

1.Copy chromatogram or spectrum traces you want to overlay into a trace window. For more information, see Section 2.5.2, Copying Traces from Multiple Data Files to a Window.

Hint: The copied traces display the original trace label and filename.

2.To use settings other than defaults, set attributes for the overlay if needed. See “Setting overlay attributes” on page 2-26.

3.Click the trace of interest to activate it.

NOTE: Only the active trace in an overlay is affected by processing tools. However, all traces are affected by zooming functions.

4.From the Display menu, select Overlay Traces.

Hint: A toolbar button is available to toggle between Overlay and Undo Overlay mode. See “Customizing

toolbars” on page 1-21for information. The button is located in the Graph category.

The traces are overlaid. The trace names are listed in the trace label.

2-24 Applied Biosystems