Chapter 9 Troubleshooting

9.4 Calibration Troubleshooting

Table 9-6 Calibration Troubleshooting—Mariner and Voyager

 

 

Symptom

Possible Cause

Action

 

 

 

 

 

 

 

Auto Calibration is turned

Current spectrum is not

Advance to the next

 

 

on, but current spectrum

calibrated until the next

spectrum, then return to

 

 

is not auto calibrated

time the spectrum is

the current spectrum. For

 

 

 

displayed

more information, see

 

 

 

 

Section 5.4, Automatic

 

 

 

 

Calibration.

 

 

 

 

 

 

 

During calibration, the

The calibration routine

When specifying highly

 

 

software is not matching

checks peak width to

charged, non-isotopically

 

 

spectrum masses to some

determine if a peak

resolved species with

 

 

reference masses in the

matches a Resolved

peaks less than 1 Da wide

 

 

calibration reference file

Isotope Mass or an

(for example, myoglobin

 

 

 

Average mass. If narrow

+20) as reference masses

 

 

 

peaks are specified as

in a calibration reference

 

 

 

Average Masses in the

file, set the peak type as a

 

 

 

calibration reference file,

Resolved Isotope Mass

 

 

 

 

 

 

the software mistakes

(even though it is not a

 

 

 

these narrow peaks as

resolved isotope). See

9

 

 

isotopically resolved and

Section 5.3.3, Creating or

 

 

ignores the reference

Modifying a

 

 

 

mass.

Calibration Reference File

 

 

 

 

(.REF).

 

 

 

 

 

 

 

 

Mass tolerance or

Adjust. See Section 5.3,

 

 

 

Minimum Intensity set too

Manual Calibration.

 

 

 

high

 

 

 

 

 

 

9-10 Applied Biosystems