Chapter 3 Peak Detection and Labeling

3.2.2 Strategy for Voyager Peak Detection

This section gives some quick suggestions on how to approach Voyager peak detection. For details on peak detection, see Section 3.2.3, Setting Peak Detection Parameters.

Default peak detection values are listed in Section 3.7, Default

Peak Detection Settings.

Strategy When detecting peaks in Voyager data:

1.Open the data file and observe the effects of the default resolution-dependent settings.

Hint: To improve peak detection, you can calculate the resolution on a tall, resolved peak in the middle of the spectrum. If the resolution result differs by more than 50% from the default resolution setting, fine-tune the

3Resolution setting and reapply peak detection. For more information, see Section 6.3, Using the Mass Resolution Calculator.

2.To aid in peak interpretation, do all of the following:

•Baseline-correct— The Centroid% peak detection value is derived from a percentage of the peak height, which is measured from 0. For information, see Section 5.8.2, Using Baseline Correction.

•Noise-filter or smooth— Use the method appropriate for your data to remove noise spikes:

Data Type

Method

 

 

Linear

Default or Gaussian smoothing

 

 

Reflector

Noise Reduction

 

 

For more information, see Section 5.7, Noise

Filtering/Smoothing.

3-8 Applied Biosystems