Chapter 5 Examining Spectrum Data

5.12Adding and Subtracting Raw or Processed Spectra from the Same or Different Data Files (Dual Spectral Trace Arithmetic)

The Dual Spectral Trace Arithmetic function lets you add two spectra together, or subtract one spectrum from another. Spectra can be raw or processed, and can be from the same or different data files.

To use the Dual Spectral Trace Arithmetic function:

1.

To perform this function on spectra from different data

 

 

files, copy a spectrum trace from one data file to

 

 

another. See Section 2.5.2, Copying Traces from

 

 

Multiple Data Files to a Window, for information on

 

 

copying traces.

2.

Activate the Spectrum window that contains the two

 

 

traces of interest.

3.

Process the traces as needed.

4.

Click the first trace in the Spectrum window to make it the

 

 

active trace.

 

 

 

 

 

NOTE: The Dual Spectral Trace Arithmetic command is

 

 

dimmed unless the first trace in the Spectrum window is

 

 

the active trace.

 

 

 

 

5.

From the Process menu, select Dual Spectral Trace

 

 

 

Arithmetic.

5

 

The Dual Spectral Trace Arithmetic dialog box is

 

displayed (Figure 5-23).

 

 

 

5-64 Applied Biosystems