HP Data Explorer 4 Labeling peaks, Creating a calibration reference file (.REF)

Chapter 6 Using Tools and Applications

Labeling peaks Click Label Peaks. The ion peaks specified in the Options

6dialog box are labeled on the trace if they are present (Figure 6-19).

Hint: To screen out labels, decrease the Label Tolerance in the Options dialog box.

Figure 6-19 Labeled Ion Fragmentation Peaks for

Synthetic Peptide (PPPPPPPPPPPPAR)

Creating a calibration reference file (.REF)

Hint: The Label Peaks function creates User Labels in the data file. To view, select Peak Label from the Peaks menu, then select User Label Setup. Click Save As to save the labels in a .LBS file for use with other data files. For more information, see Section 3.5.3, Setting Custom Peak Labels.

To display the original labels, select Peak Label from the Peaks menu, then deselect User Labels.

To create a PSD calibration reference file, click Create Reference File. For more information, see “Creating PSD Calibration Reference (.REF) Files” on page 8-21.

6-30 Applied Biosystems